DNA fingerprinting patterns of Candida species using HinfI endonuclease.
نویسندگان
چکیده
منابع مشابه
Site-dependent cleavage of pBR322 DNA by restriction endonuclease HinfI.
Cleavage of pBR322 DNA I by the restriction endonuclease HinfI is preferentially inhibited at specific HinfI cleavage sites. These sites in pBR322 DNA I have been identified and ordered with respect to the frequency with which they are cleaved. The HinfI site most resistant to cleavage in pBR322 DNA I is unique in that runs of G-C base pairs are immediately adjacent on both sites. Two different...
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A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active form. The protein binds to supercoiled DNA molecules containing its recognition s...
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We compared HinfI restriction endonuclease-based analysis of genomic DNA with a PCR-based method for molecular typing of 90 Candida krusei isolates from 17 geographically related patients. Strain groupings by these methods were the same for 89 of 90 isolates. Ten of 17 patients were infected with related strains of C. krusei.
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A number of genetic fingerprinting methods have evolved to analyze the population structure and to perform epidemiological and etiological studies of infectious fungi. These methods include multilocus enzyme electrophoresis, restriction fragment-length polymorphism using complex probes, random amplification of polymorphic DNA, and multilocus sequence typing, which are described in this chapter.
متن کاملCytosine methylated DNA synthesized by Taq polymerase used to assay methylation sensitivity of restriction endonuclease HinfI.
We have studied the resistance of cytosine methylated DNA to digestion by the restriction endonuclease HinfI, using a simple PCR procedure to synthesize DNA of known sequence in which every cytosine is methylated at the 5 position. We find that HinfI cannot digest cytosine methylated DNA at the concentrations normally used in restriction digests. Complete digestion is possible using a vast exce...
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ژورنال
عنوان ژورنال: International Journal of Systematic and Evolutionary Microbiology
سال: 2000
ISSN: 1466-5026,1466-5034
DOI: 10.1099/00207713-50-3-1381